The CDSA2.0 evaluates digestion, absorption, gut flora, and the colonic environment including immunology and inflammation. The “gold-standard” analysis of digestive function and gut microbial ecology offers comprehensive non-invasive risk assessment for colorectal cancer, differential diagnosis between inflammatory bowel disease and irritable bowel syndrome, and an assessment of exocrine pancreatic function. The profile is indicated for all chronic GI problems, acute bowel pattern changes, and for many systemic diseases, and provides a sensitivity panel for treating pathogenic flora.